Comparative study of Klebsiella pneumoniae in mice immunized with two types of antigens
Suroor Abdulkadir Tahir Al-Lamy and Zainab Razzaq Zghair
This study was designed to explor, detect the humoral immune response by using Elisa technique, to detect the cellular immune response by Delayed Type Hypersensitivity "DTH" (skin test). And to Study histopathological changes in laboratory mice organs which immunized with two prepared Ags and challenged later with viable virulent K. pneumoniae. The immunized groups (WSK Ag & WHKK Ag) revealed significant increase (p<0.05) in the mean values of (O.D) & serum (Ab.) titer level as compared with control negative group, while showed non-significant increase (p>0.05) in mean values of (WSK Ag) as compared with (WHKK Ag). All groups of mice (immunized & control negative) were conducted in (DTH) skin test at (zero time, 24, 48 & 72hrs.) and both immunized (SWHKK & SWSK Ags) groups showed positive reaction after (24, 48 & 72 hrs.) of skin test as compared with control negative group which give negative reaction. The pathological examination of a control group was infected with Klebsiella pneumonae appeared more lesions than the groups that were immunized with (WSK & WHKK) Ags. It was concluded that the (WSK Ag) was provided a good immune response better than (WHKK Ag) against the K.pneumoniae infection, also the encapsulated K.pneumoniae has the ability to dissemination and colonization of most internal organs and the inflammation was rapidly convert from acute to chronic stages.