Measurements of Pregnanediol-3-glucuronide and urinary parameters in cyclic and early pregnant Murrah buffaloes
Satya Prakash Mohapatra, SD Ingole, SV Bharucha, AS Nagvekar, PM Kekan and SD Kharde
Sixteen healthy Murrah buffaloes aged 5 - 6 years were divided into two groups: Cyclic/Non pregnant and Pregnant, comprising of eight animals each. Urine samples were collected at an interval of seven days in non pregnant group i.e day 0/day of estrus, day 7, day 14, day 21, day 28, day 35 and day 42 as well as in pregnant group i.e day 0/day of estrus/day of AI, day 7, day 14, day 21, day 28, day 35, day 42, day 49 and day 56 respectively. The PdG concentration in both the groups increased significantly (p < 0.01) on day 7 and on day 14. It declined significantly (p < 0.01) on day 21 and day 42 i.e at the end of subsequent estruses in non pregnant animals indicating luteal regression. However, the concentrations significantly increased (p < 0.01) from day 42 to day 56 with a modest decrease on day 28 in the pregnant animals accounting for the presence of persistent CL. The urine samples were absent for blood, nitrites and bilirubin. Negative to traces of leucocytes were observed which could be due to contamination of urine by vaginal discharge. The concentration of urobilinogen in all the samples was within the normal range (3.2 µmol/l to 16 µmol/l). All the urine dipstick parameters were independent of estrus and pregnancy and showed no overall correlation among them.