The present experiment was conducted to evaluate the efficacy of new molecules as well as biopesticides as foliar application against major sucking pests and toxicity against predators of these pests of Bt cotton at Cotton Research Unit Farm, Dr. Panjabrao Deshmukh Krishi Vidyapeth, Akola during 2015-2016 with eight treatment and three replications. Overall, three sprays were carried out and thus, the data obtained revealed that, newer molecule flonicamid 50% WG @ 100 g a.i./ha was found promising to managed the major sucking pests of Bt cotton followed by flonicamid 50% WG @ 75 g a.i./ha, buprofezin 25% SC @ 250 g a.i./ha and diafenthiuron 50% WP 300 g a.i./ha. However, during the present studies no deleterious effect of insecticidal treatments was observed on population of natural enemies. Highest seed cotton yield (1681.02 Kg/ha.) was obtained from flonicamid 50% WG @ 75 g a.i./ha followed by flonicamid 50% WG @ 100 g a.i./ha (1627.31 Kg/ha.). Next effective treatments were diafenthiuron 50% WP @ 300 g a.i./ha, buprofezin 25% SC @ 250 g a.i./ha, NSKE 5 % and V. lecanii @ 5 gm/lit.