author(s)
Bilal Ahmad Mir, Rahil Razak, Aarif Ali, Showkeen Muzamil, Manzoor-ur Rehman Mir, Tasaduk Khaliq, Omer Khalil Baba, Muneeb-U- Rehman, Sheikh Bilal, Ishraq Hussain and S Mudasir Rashid

abstract
Mastitis is a major threat to dairy industry which causes significant economic losses globally. The compromised antioxidant defense system is the primary factor responsible for various diseases in cattle including mastitis. The present study was designed to study the lipid peroxidation (LPO), blood urea nitrogen along with the enzymatic activities of Alkaline phosphatase (ALP), Lactate dehydrogenase (LDH), Superoxide dismutase (SOD), Catalase (CAT) and glutathione peroxidase (GPx) in the plasma samples from subclinical and clinical mastitis affected dairy cows. A total of 200 animals was selected for the study, 50 were suffering from subclinical mastitis, 30 from clinical mastitis and 120 were normal healthy cows. Blood plasma was used for biochemical and enzymatic estimation. Our study shows that ALP, LDH and LPO concentration were found significantly higher in subclinical and clinical mastitis than normal healthy cows. There was no significant difference observed in BUN levels. The mean activities of SOD, CAT and GPx were significantly reduced in subclinical and clinical mastitis than normal healthy cows. The study demonstrated that there is a significant reduction in the activities of SOD, CAT, GPX and marked increase in ALP and LDH concentration in both subclinical and clinical mastitis.