Detection of Fasciola hepatica in Abu-Ghraib district (Iraq)
Khalid Ismael Oleiwi, Zaid Salah Hussein and Kefah Oda Salman
The objective of this investigation was to determine the seropositivity of Fasciola hepatica in local sheep selected from Abu Ghraib district/ Baghdad province. To conduct such purpose fecal and blood samples of 220 animals were randomly collected from those sheep reared at the studied area during the period extended between August 2016 and March 2017. Samples were transported directly to the Lab of clinical pathology at college of Veterinary Medicine to perform some important tests that were ELISA, hemogram and leukogram evaluation, fecal examination, serum albumin, total protein and gamma-glutamyltranspeptidase. Out of the 220 samples, sera of 28 (12.73%) animals were found seropositive toward Fasciola hepatica post applying ELISA test, but only 6 (2.73%) of these animals exhibits eggs of Fasciola in their feces. Outcome of the hemogram showed significant variations (P<0.05) between seropositive and seronegative animals in the values tested such as Hb, MCV and MCHC. On the same line, the leukogram showed significant variations (P<0.05) in the tested values of WBC, Neutrophil, Eosinophil and Basophile. In another aspect, results of the biochemical tests showed significant variations (P<0.05) in albumin, total protein and GGT values between seropositive and seronegative.