Immuno-chromatographic rapid tests (FASTest® Strips) were used for the detection of Bovine, rotavirus, which gave us 159 positive results, while when we took the 159 positive samples and tested by ELISA (cosabio) kit gave us 111 positive results. Extraction of RNA and PCR methods from these 111 positive samples for detection Rota virus using vp7, vp4 primers were done and gave us 19 positive samples, The nucleotide sequence of the VP7 gene of the strains which we got were X57852.1, X52650.1, KP013395.1 & AB457636.1 from the buffalo and cattle calf feces most closely identity to that American G10 P(11), Maxico, UK G10 P(5) and Iranian G10 bovine rotavirus genotype