author(s)
Amitosh Kumar, GC Gahlot, Rajeev Joshi, Mohammad Ashraf and Subha Ganguly

abstract
One of the major genes is CYP19 gene which is related to reproduction and production traits in livestock. This study aimed to evaluate the genetic polymorphism within CYP19 gene in Rathi using PCR-RFLP Technique. Genomic DNA extracted from 60 health Rathi Cattle and was amplified using primer that were designed from the cattle CYP19 gene Sequences. The polymorphism in CYP19 gene was detected using PCR-RFLP method and PvuII restriction enzyme. AA (39 %), AB (31.66%), and BB (3.33 %). The allele was more frequent (0.81) than B (0.19). In the population it was found deviations from HWE.