Characterization of alkaline phosphatase activity in genotype of whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae)
Archana Anokhe, Kailash Chandra Naga, Sakshi Gandotra and S Subramanian
Whitefly, Bemisia tabaci (G) causes severe damage to crops directly by sucking and indirectly as vector of several plant viral diseases. It was hypothesized that physiological superiority of B-biotype in B. tabaci could partly be attributed to its improved ability to utilize the alkaline phosphatase pathway. Alkaline phosphatases (ALP) are considered as key metabolically active enzyme ubiquitously present in diverse group of insects and are functionally implicated in several physiological processes. The present study we have characterize the alkaline phosphatase (ALP) in Asia I and Asia II-1, the two predominent genotype of B. tabaci collected from different agroclimatic zone of India. The study was conducted in the year 2013-2015 in controled environment of insect proof climatic control chamber, Division of entomology-IARI, New Delhi. From the result it was found that at pH-7, Asia II-1 has shown significantly higher specific activity (0.99±0.02 nmol/µl/µg), as compare to Asia I, 0.93±0.02nmol/µl/µg which has implicated with higher Vmax- i.e. 1.338±0.141 and Km- 0.394±0.1247 nmol whereas Asia I have shown lower Vmax- 1.021±0.044 and Km- 0.1826±0.036. In this experiment we also tested the effect of temprature and pH on enzyme activity in both the genotype at optimum substrate consentration of 0.75 mM.