author(s)
Harshit Verma, Shriya Rawat, Nishant Sharma, Vikas Jaiswal and Rajeev Singh

abstract
Increasing antimicrobial resistance has become a big challenge worldwide. Mastitis is the most common disease for antibiotic use in dairy herds and thus, antimicrobial resistance of mastitis pathogens has received recent attention. The objective of the current study was to assess the status of bovine mastitis in and around Meerut region, bacterial pathogen involved and to determine the susceptibility of different antibiotics. A total of 85 milk samples were presented to Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Meerut from April, 2016 to June, 2017 with a history of swelling in udder, loss of milk, flakes formation in milk, RBC in milk and watery milk. The milk samples were cultured on brain heart infusion agar, MacConkey’s agar, eosin methylene blue agar and sabrourad dextrose agar. The isolated organisms through microbiological procedures were subjected to antimicrobial susceptibility test by disc diffusion method to a twelve number of antibiotics. The major prevalent pathogens isolated were Staphylococcus Spp. (42.55%), E. coli (21.28%), Streptococcus spp. (6.38%), Proteus spp. (8.51%), Candida spp. (2.88%) and mixed infection (18.26%). The present results revealed that gentamicin (65.96%) was the most effective antibiotic followed by enrofloxacin (63.83%), cefotaxime+clavulanic acid (52.13%), amoxicillin+sulbactum (42.55%), ciprofloxacin (41.49%), colistin (41.49%), chloramphenicol (39.36%) and ampicillin+sulbactum (38.29%). Least effective drugs were oxytetracycline (22.34%), streptomycin (25.53%) whereas maximum resistance drug were found amoxyclave (8.51%), and ampicillin/cloxacillin (8.51%).