Isolation and profiling of proteins of bacillus spp. and their influence on Meloidogyne incognita
Tamalika Sarangi, S Ramakrishnan and S Nakkeeran
Indigenous endophytic five isolates of Bacillus weihenstephanensis (TSB4), B.cereus (CLB2D), B. subtilis (TSB5), B. cereus (TSB4D), B. licheniformis (TSB4) possessing antinemic/antifungal property were assayed for the presence of different proteins through SDS-PAGE analysis. The analysis showed the presence of four proteins with different molecular weight in all the isolates of Bacillus spp. Further 2D page analysis of the proteins of B. weihenstephanensis (TSB4) identified as the most effective isolate among the five isolates exhibited both up regulated and down regulated proteins with molecular weight ranging from 14 to 96 kda. The crude proteins of all the five indigenous isolates of Bacillus had a significant effect to inhibit egg hatching and to cause mortality of juveniles of M. incognita with positive correlation between their antinemic effect and concentration time as well as period of exposure to crude antibiotics. Of all the five isolates the B. weihenstephanensis (TSB4) crude antibiotics exhibited the highest ovicidal and larvicidal effect over root knot nematode and it was differing significantly from other isolates.