Haematological changes in fresh water fish rosy barb Pethia conchonius exposed to an agrochemical calcium carbide
Sana KM Patel and ST Indulkar
An attempt has been made in the present investigation to determine the acute toxicity of calcium carbide on the widely popular ornamental fish rosy barb Pethia conchonius. Short-term acute toxicity test was performed adopting renewal bioassay technique over a period of 96 h, using different concentrations (0, 0.1, 0.01, 1.0, 10, 50 and 100 mg1-1) of calcium carbide to the fish. The 96 h LC50 value of calcium carbide to rosy barb Pethia conchonius for 96 h was found to be 57.5 mg1-1. In sublethal concentrations (1/10th i.e. 5.75 mg1-1 and 1/5th i.e. 11.51 mg1-1) fishes were exposed for 7th, 14th, 21th and 28th days and results showed significant changes (p<0.05) in haematological parameters as compared to the control. RBC counts significantly decreased from 1.540±0.001 on 1st day to 0.563±0.002 on 28th day; Hb significantly decreased from 2.211±0.015 on 1st day to 0.871±0.020 on 28th day and Hctsignificantly decreased from 21.587±0.026 on 1st day to 10.846±0.019 on 28th day. Similarly MCV, MCH and MCHC shows significantly decreasing trend. Whereas, WBCsignificantly increased from 50.030±0.033 on 1st day to 82.131±0.171 on 28th day of the study. All the estimated parameters showed significant alteration during exposure period indicated toxicity of calcium carbide to the rosy barb fish.