Gene detection of Helicobacter-pylori by use real-time PCR in patients from Wasit province: Iraq
Hassan S Jabbar and Dr. Adulbasit Adulsamed
The present study was conducted to detect the Cytotoxin–associated gene A (CagA) specific for Helicobacter pylori by using PCR technique in gastric biopsy of patients in Wasit province at OGD (esophago gastroduodenal scope) Unit at Al-Zahraa Teaching Hospital in Wasit province upper endoscopy for the period from November 2016 to February 2017. Helicobacter pylori colonization with has been recognized as an important risk factor for a gastroduodenal disease accordingly, collected from 40 adult patients, 23 men and 17women, whose age range from 13 to 72 years suffer from dyspeptic symptoms. Referred to the OGD (esophago gastroduodenal scope) Unit at Al-Zahraa Teaching Hospital in Wasit province upper endoscopy for the period from November 2016 to February 2017. According to endoscopic diagnosis, the patients were grouped into gastritis, inflammation of the duodenum, stomach ulcers. Results of analysis of biopsy samples (in size 300mg) by polymerase chain reaction (RT PCR) as invasive procedure of the 40 enrolled patients were subjected for Cag-A gene, result showing seventy-five (75%) (30) patients exhibited positive results to Cag-A gene, also show various ratio in gastritis, gastritis & duodenitis, and gastropath ulcer with positive samples for qPCR-CagA, were ratio (40%), (85.71%) and (87.5%) respectively, the difference in the distribution of H. pylori CagA positive with sex at being nonsignificant (P> 0.05), also no significant was found between CagA and age (P> 0.05).