Evaluation of different preservative media upon integrity and activity of abattoir ram caudal spermatozoa
Dr. Wafir Mahdi Saleh
The present study is designed to evaluate different preservative media that used in the collection of caudal spermatozoa from abattoir specimens. Seventy abattoir testicles samples are collected from Al-Shoáalla slaughterhouse North-West of Baghdad during the period from November 2015 to April 2016. The total number is divided into seven aliquots (ten samples each). For each aliquot one of the seven media (N. saline, MEM, TCM-199, Glucose, MEM+ N. saline, TCM-199+ N. saline and Glucose+ N. saline) is used to estimate the dead, alive and motile spermatozoa. Time between slaughtering and processing in the lab and effect of transport temperature also studied. In conclusion the differences were not significant among the different media in which normal saline is the cheapest and more practical one. The period among samples collection and lab processing must be limited to minimal period. The more suitable temperature upon specimens transport is between 4-8°C.