author(s)
Mandeep Kaur, KK Chahal, Amit Kumar, Ramandeep Kaur and Urvashi

abstract
In vitro studies were conducted to evaluate the nematicidal activity of bay leaf essential oil, its fractions, isolated and derivatized compounds for egg hatching and juvenile mortality of second stage juveniles of M. incognita at different concentrations (0.20-1.50 mg mL^-1) and durations (24-96 hrs) in Department of Plant Pathology, Punjab Agricultural University, during the year 2016-17. Bay leaf essential oil was partitioned into non-polar and polar fractions. Eugenol, 1, 8-Cineole and α-Pinene were isolated using column chromatography. α-Pinene and eugenol were chemically derivatized into campholenic aldehyde and eugenol epoxide respectively using metachloroperbenzoic acid. Bay leaf essential oil, its fractions, α-Pinene, 1,8-Cineole, eugenol, eugenol epoxide and campholenic aldehyde showed effective egg hatch inhibition and increased juvenile mortality at all concentrations and durations. Among the various components tested, bay leaf essential oil showed highest egg hatch inhibition and mortality. It showed complete egg hatch inhibition at 1.00 mg mL^-1 after 72 hrs and complete mortality was observed at 0.80 mg mL^-1 after 96 hrs. Both the activities were concentration and time dependent. The present studies revealed that bay leaf essential oil is effective against M. incognita and can be explored further for its nematicidal properties.