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Journal of Entomology and Zoology Studies
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ISSN Print: 2349-6800 | ISSN Online: 2320-7078

Journal of Entomology and Zoology Studies

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2018, Vol. 6, Issue 2
Larvicidal, ovicidal and repellent activities of Streptomyces enissocaesilis (S12-17) isolated from Western Ghats of Tamil Nadu, India

Pathalam Ganesan, Sekar Anand, Subramanian Sivanandhan, Rajendran Host Antony David, Micheal Gabriel Paulraj, Naif Abdullah Al-Dhabi and Savarimuthu Ignacimuthu

The aim of the present study was to isolate Streptomyces enissocaesilis (S12-17) from the soil sample collected from Venganayakkan region in Western Ghats of Tamil Nadu, India and check for its mosquito larvicidal, ovicidal and repellent activities. Isolation was performed by serial dilution using spread plate technique. Extract from S. enissocaesilis was checked for mosqutiocidal activity by using standard methods. Ethyl acetate and methanol extract presented good larvicidal activity with LC50 and LC90 values of 207.18, 635.21 and 282.30, 894.13 respectively, at 500ppm concentration against Cx. quinquefasciatus. Ovicidal activity of ethylacetate crude extract was 100% and methanol extract was 89% at 500ppm concentration against Cx. quinquefasciatus. The ethyl acetate extract gave repellency of 127.2 min and the methanol extract gave repellency 80.6 min at 5mg/cm2 concentration against Cx. quinquefasciatus. Both the extracts was harmless to tested non-target organism P. reticulata The active strain S12-17 was identified using 16S rRNA sequencing; phylogenetic tree was constructed using MEGA4 software; the sequence was submitted in the GenBank (Accession No. KT827105.1). The present study clearly showed that the extracts from S. enissocaesilis were effective in controlling mosquito life stages. The mosquitocidal activities deserved in this study, encourage the search of new insecticides having better properties.
Pages : 1828-1835 | 388 Views | 24 Downloads
How to cite this article:
Pathalam Ganesan, Sekar Anand, Subramanian Sivanandhan, Rajendran Host Antony David, Micheal Gabriel Paulraj, Naif Abdullah Al-Dhabi, Savarimuthu Ignacimuthu. Larvicidal, ovicidal and repellent activities of Streptomyces enissocaesilis (S12-17) isolated from Western Ghats of Tamil Nadu, India. J Entomol Zool Stud 2018;6(2):1828-1835.
Journal of Entomology and Zoology Studies
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