In experiment two, investigate the analgesic and anti-inflammatory effects of DI aqueous leave extract in four treated groups each of 6 animals that consist of two DI extract treated gatherings (T1&T2) orally dosed with DI leaves aqueous extract at 100 and 200mg/Kg BW. compared with a group dosed Diclofenac (0.71mg/Kg BW)and Meloxicam(0.3mg/kg). The fourth group acts as a control one. in indicated that there were significant analgesic effects in dose dependent manner that means the extract possibly has central and peripheral effects. The high DI dose showed nearly the same analgesic effectas Diclofenac at dose 0.71mg/kg, T2 group and Diclofenac (0.71mg/Kg BW) group (6.7 ± 0.2 and 7.6 ±0.2) respectively after 30 minutes of administration and the effect of analgesia continued in both T2 and Diclofenac group (6. 9 ± 0.18 and 6.3 ± 0.3) respectively at 60 minutes after gives and effect appeared at 90 minute T2 and Diclofenac group (5. 7 ± 0.2 and 5.8 ± 0.12). while T1 showed pain reaction time of (5.8 ± 0.2 and 5.8 ± 0.12 and 4.6 ± 0.3) respectively at 30, 60, 90 minutes revealed less effective than T2 and Diclofenac group and all these three groups were in significant comparison with the control group that in pain reaction time (4.2 ± 0. 2 and 4.4 ± 0.1 and 4.3 ± 0.2) in different periods.

The results appear caused a significant decreasing (P˂0.05) T1 (54 ±2.5) T2 (37± 3.2) and Diclofenac (0.71mg/Kg BW) (29 ± 1.2) comparison with the control group (70±1.5) in writhing test. In formalin test the results showed that both D. innoxia doses in early and late phase T2 (25.0±1.0,12.10±1.30) T1 (30±1.2,20.1±1.55) Meloxicam (0.3mg/kg) (24.7±1.0,11.10±1.20) and control (38.5± 2.20, 39±2.10) recorded their analgesic and anti inflammatory effects, this is probably due to the active phytochemical contents that posses both analgesic and anti-inflammatory effects.