Effect of acellular bovine pericardium and dermal matrixes on cutaneous wounds healing in male rabbits: Histopathological evaluation
Ahmed HF AL-Bayati and Falah Mahmood Hameed
The present study was designed to evaluate the effects of two types of natural materials (Biomaterials), acellular bovine pericardium (BP) and acellular dermal matrix (ADM) on full-thickness cutaneous wounds healing in male rabbits. The animals were housed in metal cages in the animal house of the Veterinary Medicine College, Baghdad University, along the period of the study 35days from 4 March to 10 April of 2017. A total of seventy five (2X2) centimeters of square full-thickness cutaneous wounds were created in 50 male rabbits under the effect of I/M administration of a mixture of 5mg/kg of xylazine hydrochloride and 35mg/kg of ketamine hydrochloride. The dorsal aspects (back) of lateral thoracic side of animals were prepared for aseptic surgery. Two wounds were made on one side of the back, the caudal one as treatment wounds, and the cranial as control wounds with a distance of (5-7) cm between each. Depending on the method of the treatment, these wounds were divided into three groups (A, B and C) (25 wounds/group). In group (A), the caudal wounds were treated immediately by covering of wound bed with a sheet of ADM, and in group (B), the wounds were treated by covering of wounds bed with a sheet of BP, while in group (C), the cranial wounds were left without treatment, as a control wounds. For histopathological evaluation, each group was divided into five subgroups (five wounds/subgroup). The histopathological studying on (3, 7, 14, 21 and 35) days post-treatment revealed that BP, ADM treated wounds have enhanced cellularity, increased vasculature than those in untreated wounds. Depending on the histopathological findings, this study confirms that local implantation of xeno-grafting BP and ADM leads to enhance and develop of cutaneous wound healing.
Ahmed HF AL-Bayati, Falah Mahmood Hameed. Effect of acellular bovine pericardium and dermal matrixes on cutaneous wounds healing in male rabbits: Histopathological evaluation. J Entomol Zool Stud 2018;6(2):1976-1986.