The present study was carried out from March 2014 to February 2016 at Bareilly district, Uttar Pradesh to explore the ichthyofaunal diversity of the middle stretch of the Ramganga River. A total of 58 fish species belonging to 9 families and 7 orders. According to the IUCN status, 5 species are endangered, 15 vulnerable, 16 low risk, 6 least concern, 10 not evaluated and 6 exotic. The Cyprinidae family with 21 representative species was found to be dominant (36%) in the present study followed by Bagridae with 6 species (10%) and Siluridae with 4 species (7%). Our study result reveals that the river supports considerable diversity of the fishes and is important for conservation as some fish fauna is threatened being either vulnerable or endangered. However strategies such as sustainable harvesting, control of growth of exotic species, check on water pollution and regulation of destructive fishing methods are suggested for conservation of the native and endemic fish species of the river.