Identification of pathogen causing common bacterial blight (CBB) of bean through the biochemical and molecular pathway and their management system
Razia Sultana, Md. Samiul Islam, Md. Asadul Islam and Biswanath Sikdar
The present laboratory assays were done in September 2017 to identify the causal agent of Common Bacterial Blight (CBB) disease in bean (Phaseolus vulgaris L.) and its management options. The disease caused by the gram-negative bacterial pathogen Xanthomonas axonopodis pv. phaseoli. The growth characteristics were determined on the basis of appearance, color, size, and shape of colonies. Among the different biochemical tests, some showed a positive reaction against the isolated bacteria while rest of showed negative results. An antibiotic sensitivity test was done to control the isolated bacteria where potential results were found. To control the isolated bacteria antagonistic assay has been done using four isolated soil bacteria where no potentiality was found. PCR was performed with the specific primer pairs, 27F and 1319R which direct the amplification of the 1250-bp DNA fragment. This study assists to confirm that the investigated strains belong to species Xanthomonas axonopodis pv. phaseoli and its management options.
Razia Sultana, Md. Samiul Islam, Md. Asadul Islam, Biswanath Sikdar. Identification of pathogen causing common bacterial blight (CBB) of bean through the biochemical and molecular pathway and their management system. J Entomol Zool Stud 2018;6(3):752-757.