author(s)
Rahil R Bhat, Insha Amin, Aarif Ali, Bilal A Mir, Sanna Bashir, Nazira Bashir, Sheikh Bilal Ahmad, Omer Khalil Baba, Showkeen Muzamil, Pervaiz Reshi, Ishraq Hussain, Bilquis Fatima, Muneeb ur Rehman and Manzoor R Mir

abstract
The current study, carried between June 2017 to March 2018, was directed in Holstein Frisian dairy cattle under second lactation with an aim to evaluate bovine hs-CRP secretions in the milk and serum of normal and subclinical mastitis (SCM) cows after screening by CMT and milk electric conductivity (EC) test. The study includes a total of 72 cows comprising of 10 in normal and 62 in subclinical group. The mean values of SCC in normal and subclinical groups were significantly different (p≤0.001). The mean values (with standard error) of somatic cell count (SCC) in normal and subclinical groups for SCC were1.820 ± 0.148 and 4.850 ± 0.512 respectively. Quantitative analysis of bovine hs-CRP by ELISA showed significant difference between normal (26.0363±11.14) and subclinical (267.603±30.17) serum samples while as no evident results were seen in milk samples of same animals.