author(s)
Om Prakash Dinani, Pramod K Tyagi, Nasir Akbar Mir, AB Mandal, Praveen K Tyagi and Sita Prasad Tiwari

abstract
A biological experiment was conducted to evaluate the effect of feeding rice distillers dried grains with solubles (rDDGS) and rice gluten meal (RGM) combination as a replacement of soybean along with exogenous enzyme supplementation on the intestinal morphometry and microbiology of broiler chicken at 21 days of age. Following a 2x3 factorial design, the experimental diets were: T1 (no rDDGS/RGM/enzyme), T2 (no rDDGS/RGM, with multienzymes), T3 (12.5% rDDGS, 15% RGM, no enzyme), T4 (12.5% rDDGS, 15% RGM, with protease enzyme), T5 (10% rDDGS, 12.5% RGM, no enzyme), and T6 (10% rDDGS, 12.5% RGM, with protease enzyme). Each treatment was allocated 5 replicates of broiler chicken with 8 birds in each. The feeding of the dietary combination of 12.5% rDDGS and 15% RGM significantly (P<0.05) decreased the villus height (VH), crypt depth (CD), VH: CD ratio and villus width compared to the control and other rDDGS & RGM combination. The microbiological parameters revealed that TVC in crop and jejunum were increased significantly (P<0.01) in 12.5% rDDGS & 15% RGM as compared to control and other dietary combination. Lactobacillus count in crop was decreased significantly (P<0.05) in12.5% rDDGS & 15% RGM as compared control. It was concluded that the simultaneous inclusion of rDDGS and RGM in broiler chicken ration with or without enzyme supplementation had adverse effects on the intestinal histomorphometry and microbiology.