Biological characterization of different isolates of Pieris brassicae Granulosis virus
Ramandeep Kour, RK Gupta, Hafeez Ahmad, Suheel Ahmad Ganai, Nadeya Khaliq and Simranjeet Kour
To determine the biological characteristics of different isolates of Pieris brassicae Granulosisvirus, bioassays were carried outagainst the third instar larvae of Pieris brassicae under laboratory conditions. The four different GV isolates of P. brassicae isolated from different geographical regions, exhibited differential degree of pathogenicity in terms of both LD50 and ST50. The LD50 values of different GV isolates of P. brassicae against 3rd instar larvae of P. brassicae varied from 6.31 x 103 to 1.22 x 104 OBs/larvae with non significant differences between the isolates. However, significant differences were observed in the ST50 estimates of four different isolates of PibrGV against 3rd instar larvae of P. brassicae which varied from 5.90 to 7.43 days. The Sudhmahadev isolate exhibited 11.01- 20.59% reduction in ST50 as compared toPoonch, Chatha and Himachal Pradeshisolates. Among all these isolates, Sudhmahadev isolate of PibrGV was found to be most effective.