Antibiotics are used as chemotherapeutic, prophylactic purposes and also as feed additives to promote growth and improve feed efficiency in poultry industry. The present study was conducted to evaluate the antimicrobial sensitivity and multidrug-resistance for Salmonella species isolated from broilersof Hoshagabad districts of Madhya Pradesh. A total of 153 samples consisting of cloacal swab (99), dry faeces (18), feed (18) and water (18) of all the three seasons were collected. Phenotypically 13 isolates were confirmed as Salmonella species. Genotypically all the isolates were positive for 16S rRNA and invA gene in polymerase chain reaction. Out of the 13 isolates 69.23 isolates were positive for Salmonella enterica ser. Typhimurium gene. In antibiogram the isolates showing resistant pattern in decreasing order were gentamicin (11, 84.61%), norfloxacin and ampicillin / sulbactum (10, 76.92%), ofloxacin and ciprofloxacin (09, 69.23%), streptomycin and ampicillin (08, 61.53%). Antibiotics vancomycin and co-trimoxazole were sensitive for Salmonella organism. It was found that the maximum isolates were showing resistance to more than 06 antibiotics.