author(s)
G Kamila, GR Sahoo, PC Mishra, PC Behera and PR Sahoo

abstract
The present study was conducted to establish a suitable marker for diagnosis of reproductive disorder of cross bred cows by characterizing the serum proteins, at Department of Veterinary Biochemistry, College of Veterinary Science & AH, Bhubaneswar from the time period between December 2016 to October 2017. In this study, five groups of animals (each having six animals) such as repeat breeder (RBC), heifer anestrus (HA), repeat breeder heifer (RBH), post-partum anestrus (PPA) cows and normal cycling cows (NC) were taken for experimental purpose. Serum protein from each group was isolated and characterized through one dimensional electrophoresis and subsequent densitometry studies. The electrophoretic characterization revealed that there is an expression of one extra band of proteins (155 kDa) in HA and PPA, but RBH and RBC showed two extra protein bands of 34, 20 kDa and 116, 110 kDa approx. respectively. So it can be concluded that these extra band might be responsible for the reproductive failure and would be a better platform to act as a marker for diagnosis of reproductive disorder.