Antagonistic activity of bacterial endophytes against major soilborne pathogens of soybean
Brunda KS, Shamarao Jahagirdar and Kambrekar DN
The antagonistic effect of 30 bacterial endophytes of soybean collected from northern Karnataka and parts of Maharashtra against Sclerotium rolfsii, Rhizoctonia bataticola and Fusarium oxysporum were assayed in vitro through dual culture plate technique. The bacterial endophytes RB-KK-6 (40.78%), SB-BS-6 (50.08%) and LB-BU-1 (47.02%) were found effective against S. rolfsii and the isolates SB-DG-11 (47.41%), LB-BiN-8 (41.22%) were effective against R. bataticola. The effective bacterial endophytes against F. oxysporum were RB-HS-1 (41.99%), SB-BiJ-9 (40.07%), LB-BU-1 (54.20%) and LB-BV-2 (51.64%). Based on molecular characterization the effective bacterial endophytes were identified as Acinetobacter sp. (RB-HS-1), Alcaligenes faecalis (RB-KK-6), Stenotrophomonas sp. (SB-BiJ-9), Bacillus pumilus (SB- DG-11 & LB-BiN-8), Paenalcaligenes sp. (LB-BU-1), Bacillus cereus (SB-BS-6) and Brevibacillus sp. (LB-BV-2).