Ant communities are affected by anthropogenic disturbance related to conversion of forest into agroforestry systems. The effect of this disturbance on arboreal ant diversity is poorly documented in the African agroforestry systems in general and in Cameroon in particular. In the Centre region of Cameroon, the study was done in Bokito, Ngomedzap and Nkolbisson along a latitudinal gradient ranging from evergreen forest to savanna through the ecotone. Visual sampling technique were used and sixty one ant species from twenty two Genera were identified. The most speciose Families were Myrmicinae and Formicinae, with twenty species each. Tetramorium aculeatum was the most frequent species followed by Oecophylla longinoda. The forest habitat harboured the highest diversity compared with savanna and ecotone. Ant species richness between these landscapes showed a strong evidence of variation in the diversity and occurrence although a generalized binomial linear model showed a low influence of habitat type on ant community.