Optimisation of oocyte maturation media for improving efficiency of in vitro embryo production
Shafqut Majeed Khan, Suhaib ul Haq, Abha Mariam, Aadil Majeed, Aiman Ashraf, Shakil Bhat and Riaz Ahmad Shah
In vitro maturation is essential technology under assisted reproductive technologies (ARTs) which enables oocytes to achieve maturation and acquire competence for subsequent embryonic division leading to blastocyst formation). This technique is valuable not only because they allow production of large numbers of oocytes, but also because they provide valuable in vitro model to study the gene expression at different maturational stages of oocyte. In a typical in vitro embryo production technique, either by cloning or by in vitro fertilization (IVF). IVM of oocytes constitute the most challenging step because an orchestrated gene expression events during this time determines the efficiency of fertilization and subsequent embryonic division process leading to blastocyst formation and even successful implantation. Optimisation of IVM media composition is important to increase the efficiency of in vitro embryo production. Foetal Bovine Serum (FBS) was supplemented to IVM media at 10% and 15% levels along with addition of 5 µgmL¯1pFSH, 1 µgmL¯1 17β-estradiol. The maturation percentage was found to be significantly higher when 15% FBS was used both with the addition of β-Estradiol and FSH and without the hormones (55.37± 2.61 v 79.31± 1.75 and 77.88± 1.91, respectively). Cleavage percentage was also found to be significantly higher with 15% FBS (68.88± 2.50) than with 10% FBS (55.35± 4.31) (P<0.05). Morula percentages were also found to vary in a similar fashion between three groups of IVM media, 33.97± 1.61 v 41.74± 4.31 v 39.14± 2.31, respectively, but the differences were not significant statistically at significance level of P<0.05. Similar was the case with blastocyst percentages, 4.00± 2.31 v 14.80± 3.92 v 9.08± 3.60, respectively
Shafqut Majeed Khan, Suhaib ul Haq, Abha Mariam, Aadil Majeed, Aiman Ashraf, Shakil Bhat, Riaz Ahmad Shah. Optimisation of oocyte maturation media for improving efficiency of in vitro embryo production. J Entomol Zool Stud 2018;6(6):1332-1335.