author(s)
GK Saikia, P Konch, A Boro, R Shome, H Rahman and SK Das

abstract
Brucellosis is most contagious, often regionally neglected disease of small ruminants in the country and study aimed to estimate the sero-prevalence of caprine brucellosis in Assam state in the Indian subcontinent. A total of 1514 goat sera samples were collected from organised farms located in 19 out of 33 different districts of Assam and screened by RBPT and iELISA. In addition, 33 clinical samples including vaginal discharge (2), vaginal swab (29) and aborted foetus (02) from aborting goats were processed for isolation of Brucella. Out of 1514 goat sera samples screened, 26 were found to be positive by both the tests with an overall sero-positivity of 1.72%. Seroprevalence was highest in animals above 18 months (2.30%) of age and animals having history of abortion (12.99%). Isolation from 33 clinical samples yielded one Brucella spp. from one aborted foetus. The isolate was identified by bacteriological identification tests, bcsp31 genus and species specific AMOS and Bruce ladder PCRs as Brucella abortus.