The present study reports a mycobacterium infection with the rare transmission of Mycobacterium bovis infection between an adult male dog and potentially other infected animal through close contact. Clinical signs revealed progressive weight loss, emaciation, chronic diarrhea and loss in condition. Despite receiving earlier supportive treatment, it progressed to death. Post-mortem examinations revealed presence of granulomatous lesions. Ziehl-Neelsen stains demonstrated numerous slender, slightly beaded acid-fast bacilli consistent with mycobacteria in the liver and kidneys confirmed by PCR. Positivity of tuberculosis in a dog poses a risk of transmission of infection between the dog and other animals including humans.