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Journal of Entomology and Zoology Studies
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P-ISSN: 2349-6800, E-ISSN: 2320-7078

Journal of Entomology and Zoology Studies

2019, Vol. 7, Issue 1
Effect of hormone combination on in vitro maturation of goat oocytes and its diametrical evaluation of expansion in different IVM media

Girin Hazarika, Ranjan Kumar Biswas, Prabodh Borah, Devojyoti Dutta, Nikhil Chandra Nath, Manoj Kumar Kalita and Bharat Chandra Deka

Diametrical measurement of expansion of goat oocytes (COCs) after IVM for 24 hrs, 38.50C, 5% CO2 was evaluated objectively in different media, viz., 1 to 4. The maturation based on cumulus cells expansion was highest (84.24%) in medium 4. The mean diameter (µm) of oocytes with cumulus cells before and after IVM varied significantly (P<0.05) in all media for all types of oocytes, except for C type of oocytes in medium 1. The increment in the mean diameter of oocytes with cumulus cells after IVM differed significantly (P<0.05) between all the media for B and C types oocyte. The increase in the mean diameter of COCs was significantly (P<0.05) higher in medium 4 for all types of oocytes. Increase mean diameter between all types oocyte with cumulus cells after IVM differed significantly (P<0.05) in medium 1. In medium 2 the values for C type of oocytes was significantly (P<0.05) lower than that of A and B types oocytes. The increment in mean diameter for A type oocytes was significantly (P<0.05) higher from B and C types oocytes in medium 3. In medium 4, increase in mean diameter of A type of oocytes differed significantly (P<0.05) from C type oocytes but not from B type oocytes and varied significantly between B and C types oocyte mean diameter.
Pages : 742-746 | 466 Views | 78 Downloads


Journal of Entomology and Zoology Studies Journal of Entomology and Zoology Studies
How to cite this article:
Girin Hazarika, Ranjan Kumar Biswas, Prabodh Borah, Devojyoti Dutta, Nikhil Chandra Nath, Manoj Kumar Kalita, Bharat Chandra Deka. Effect of hormone combination on in vitro maturation of goat oocytes and its diametrical evaluation of expansion in different IVM media. J Entomol Zool Stud 2019;7(1):742-746.

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