author(s)
A Venkatesh, K Kishore, G Mallikarjuna, L Satish, AV Mary Josepha and V Sivaprasad

abstract
Silkworm pebrine, caused by Nosema bombycis, it causes great losses in sericulture all over the world. Research on host response in silkworm following N. bombycis infection has given interesting results, especially pertaining to the innate immune response to the pathogens. In this present study is to investigate the silkworm heamolymph proteome profile changes after the inoculation of N. bombycis. Silkworms were explorated with N. bombycis, and analyzed the differentially expressed proteins in both infected and uninfected hemolymph. Inoculated the pathogen spores (1x10⁸) through per oral to the 5^th instar 1^st day of silkworm larvae and hemolymph were collected different time intervals after inoculation as well as control. Conducted Nano-drop spectrophotometry, electrophoresis and different staining methods for the detection of expression of protein profiling. The electrophoresis SDS-PAGE methods was used to identify differentiation of protein expression and analyzed the targeted band by using MALDI-TOF-MS and MASCOT tools for the characterization of the protein. After the inoculation hemolymph protein concentrations were up-regulated and down-regulated. The up-regulated 25 kDa protein band was targeted in hemolymph after 5^th dpi, based on its expression level at different intervals. Targeted protein was analyzed through the MALDI-TOF-MS and got the amino acid sequence. MASCOT analysis data have concluded that the targeted protein sequence matched 38% of homology with Neprilysin-3, a Metalloendoprotease protein found in Drosophila melanogaster. Results will be valuable to further study of insect innate immunity and to aid in a comprehensive understanding of the silkworm immune mechanism after inoculation of the pathogens.