author(s)
Jazem A Mahyoub

abstract
Nowadays Metallic nanoparticles is a hot topic used for cleaning waste water as well has the applications in the field of Medical sciences. In the present study, fresh leaves of Dodonaea viscosa have been used for synthesis of silver nanoparticles and its Larvicidal activity was evaluated against Aedes aegypti larvae. The biosynthesized silver nanoparticles (AgNPs) were characterized using Scanning Electron Microscopy (SEM) and Fourier Transformed Infrared spectroscopy (FTIR). Randomly amplified polymorphic DNA technique was used for the genetic toxicity of the dengue mosquito larvaes. Results revealed that LC₅₀ of AgNPs was more effective as compared to that of D. viscosa extract by 1.38 fold. Molecular assessment using three set of primers did not showed any mutation in the DNA. However the growth of larvae was greatly affected by AgNPs treatment as compared to control one. The retardation in the larval growth might be due the under expression and/or mutational effects of the AgNPs nanoparticles. In addition, the AgNPs resulted deformation in larvae stage of the A. Aegypti and also disturbed the metamorphosis of larvae to pupa and pupa to adults. Therefore, our results revealed a promising effects of D. viscosa AgNPs and could be used as ecofriendly pesticide for the control of dengue fever vector.