author(s)
AS Vaishnavi, A Mangala Gowri, C Valli, TV Meenambigai and D Baskaran

abstract
Nano particles explicitly need to be non toxic not only for biomedical applications but for other areas that ends up in environmental exposures. Various platforms are being used from in vitro cell culture to higher vertebrate models. However, small efficient, more informative but cost effective well established Zebrafish models are the choice for preclinical studies due to the advantage of sharing not less than 85 percent homology with human genome biology. Systematic vision gained by toxicity assessment of food using various models from in vitro cell-based assays, biochemical assays, in vivo animal to clinical settings paved way for a better food safety. The present study was designed to find out the comparison and toxicity between Nano Selenium (NaSe) and Inorganic Selenium (Inorg Se) on the embryonic development of Zebrafish. Different concentration of dietary Se was used on developing Zebra fish embryos. The results indicated that with the increased NaSe and Inorg Se concentration, different observable deformities such as growth retardation, shrinkage of chorion, yolk sac edema, lack of pigmentation; tail deformities and scoliosis in developing embryos were observed. The NaSe at 0.1mg/L concentration showed the highest hatchability and healthy embryo development. This study is conducted to identify the optimal NaSe concentration that could be used for food fortification through supplementation in developing zebra fish embryos.