author(s)
Keleson Basumatary, Bula Das, Probodh Borah, Luit Barkalita, Krishna Bharali and Shantanu Tamuly

abstract
Prolactin receptor (PRLR) genes are found to be associated with the molecular basis of useful production traits in poultry. The present study was conducted to study the polymorphism of PRLR gene in 101 indigenous ducks of Assam. PCR-RFLP analysis of PRLR gene using PciI restriction enzyme revealed two genotypes, arbitrarily designated as AA, AB. Following digestion of 367 bp amplified product of PRLR gene yielded two fragments (108 bp and 259 bp) of AA genotype and three fragments (108 bp, 259bp and 367 bp) of AB genotype. The frequencies of A and B alleles were found to be 0.956 and 0.045, respectively and genotypic frequencies 0.911 and 0.089 for AA and AB genotype, respectively. A variant of PRLR gene was found to be predominant in the indigenous ducks of Assam with the highest frequency of AA genotype followed by AB genotype (AA>AB). Chi-square (χ2) test revealed that the population under study was in Hardy-Weinberg Equilibrium for PRLR gene.