Detection of infectious bursal disease virus by using one-step PCR method
Preeti, Suresh Kumar, Naresh Jindal and Ramkaran
The present study was conducted to detect the infectious bursal disease virus by using one-step reverse transcription polymerase chain reaction (RT-PCR) from IBD-affected broiler flocks in Haryana, India. Of the 85 pooled bursal samples, 83 (97.64%) bursal samples were found positive for IBDV by one step RT-PCR using VP2 gene specific primers.