author(s)
Dr. Sanjita Sharma, Dr. Vishnu Sharma, Dr. Rashmi Singh, Dr. Anurag Pandey and Dr. Ankit Kumar Singh

abstract
In India, antibiotics are being used in large quantities for decades, until the existence of these drugs in the milk has received a little concern. The prolonged or inappropriate usages of antibiotics are leading to residues, which pose the risk of human health hazards and are also interfering with the processing of the milk and milk products. The separation of Oxytetracycline antibiotic was achieved on an Acclaim C18 analytical column (150 mm x 4.6 mm x 5 µm). The mobile phase was a mixture of.01M oxalic acid, acetonitrile and methanol (70:20:10 v/v) at a flow rate of 1ml/min at 30⁰C temperature. Milk samples were collected from different places of Jaipur.Antibiotic residues were detected in most of the samples. It was found that there was an increase in the residual level of milk up to three days of treatment after that the concentration of the antibiotic residue starts declining.