author(s)
Randeep Kumar, Bahaar Anjum, Ravendra Kumar, Om Prakash, Ganesh Panday, AK Pant and RM Srivastava

abstract
Present research was undertaken to investigate the chemical composition, total phenolics assay, in vitro antioxidant, anti-inflammatory and insect antifeeding activity in chloroform extract of Limnophila indica (L.) Druce. GC-MS analysis revealed the occurrence of 4,5-Dimethyl-1,2,3,6,7,8,8a,8b-octahydrobiphenylene (18.3%) as a major compound. Total phenolic content, flavanoid content and orthodihydric phenol in the extract were 150.55±0.33 mg/g GAE, 40.72±0.69 mg/g CNE and 56.66±0.93 mg/g CLE, respectively. In-vitro antioxidant activity estimation through NO radical scavenging activity, DPPH radical scavenging activity, metal chelating activity of Fe⁺³ and reducing power activity of Fe⁺³ to Fe⁺² resulted its potential result with IC₅₀ values of 35.85±3.12, 28.52±1.32, 49.29±0.15 and 39.17±0.65 respectively. Anti-inflammatory activity determined using inhibition of protein denaturation method and extract showed significant activity (IB₅₀=27.96±1.14). Chloroform extract of L. indica at the dose levels of 20% and 25% also exhibited significant antifeeding activity against Spilosoma oblique.