Mass multiplication of Metarhizium anisopliae on various substrates
Neelesh Raypuriya, SB Das, AK Bhowmick and Vibha
During the 2016-17, for the mass multiplication of Metarhizium anisopliae (Metchnikoff) Sorokin, in the laboratory of the Department of Entomology (JNKVV, Jabalpur), many natural and liquid substrates (i.e. rice, maize, sorghum, green-gram, chickpea, wheat, potato dextrose broth, sabouraud’s dextrose broth and rice starch) were used for mass multiplication of M. anisopliae, which found that the highest spore load of M. anisopliae (9.78 x 1010 cfu ml-1) was harvested from rice followed by SDB (8.11 x 1010 cfu ml-1) and chickpea (8.00 x 1010 cfu ml-1) respectively. Whereas, the minimum number of spores (2.78 x 1010 cfu ml-1) was obtained from wheat. Significantly lowest production cost was obtained in rice (Rs. 0.26 for 1 x 1010 spores’ ml-1). Therefore, rice was found to be the most appropriate and economical substrate for the mass multiplication of M. anisopliae followed SDB and Chickpea.
Neelesh Raypuriya, SB Das, AK Bhowmick, Vibha. Mass multiplication of Metarhizium anisopliae on various substrates. J Entomol Zool Stud 2019;7(2):614-616.