author(s)
Dorlikar PR, SR Warke, UM Tumlam and VC Ingle

abstract
Diarrhea is a very common condition in dogs caused by various viral and bacterial causative agents. It has economic implications when a large number of dogs are affected in quick succession. It is multifactorial and the origin of Canine parvovirus (CPV) though is not absolutely clear but phylogenetically originated from feline panleukopenia virus or a very closely related carnivore parvovirus of feral canids like foxes and mink. The current study was designated for isolation and identification of parvovirus infections in dogs as one of the most important and main viral infection causing diarrhea in and around Nagpur by testing 91 fecal samples of diarrheic dogs below three months of age from September 2017 till March 2018. The samples were inoculated in MDCK cells revealed successful isolation of rotavirus in five samples with characteristic cytopathic effects Out of 91 faecal samples 41(45.05%) samples were found positive with a uniform band of 681 bp. Among all PCR positive samples, one sample randomly selected for virus isolation in MDCK cell line, virus gets adopted after 3 passages. Characteristic cytopathic effect was noticed after 24 hours of post infection in shape of rounding of cell, followed by complete rounding of cell after 48 hours, clumping in 72 hours of post infection and eventually detachment of cell after 96 hrs. by using polymerase chain reaction (PCR).