author(s)
Anil S Kotiya, KH Vadher, AJ Bhatt and Dave TH

abstract
Culture shrimp sample of Litopenaeus vannamei were collected from commercial pond at Datardi village, Rajula (Gujarat). All pond harvested shrimp were collected in concern chill dead tank, from this, shrimp sample were randomly selected for biochemical composition. The tests were implemented three times. The present study was conducted to evaluate the effect of stocking density on L. vannamei with the emphasis on proximate, nutritional, amino acids and fatty acid composition. The proximate composition of L. vannamei was higher protein (%) in ST1 (30 nos/m²) treatment followed by crude fat (%), carbohydrate (%) and Ash (%) in ST1 (30 pc/m²). Comparing all treatment major elements like calcium content was higher in ST5 (70 pc/m²) with 24.52±2.12 than potassium (K) in ST6 (80 pc/m²), sodium (Na) in ST6 (80 pc/m²) and magnesium (Mg) in ST1 (30 pc/m²) whereas minor elements predicted were Copper (Cu) followed by iron (Fe), Zinc (Zn), Manganese (Mn) and Chromium (Cr). Totally 19 amino acids were detected, among these, arginine, histidine, isoleucine, threonine, leucine, methionine, phenylalanine, tryptophan, lysine and valine are essential amino acids and alanine, asparagine, aspartic acid, cysteine, glutamic acid, glycine, proline, serine and tyrosine are non-essential amino acids. In total essential amino acids recorded in ST4 (60 pc/m²) followed by ST1 (30 pc/m²), ST3 (50 pc/m²), ST6 (80 pc/m²), ST2 (40 pc/m²) and ST5 (70 pc/m²) whereas the total non essential amino acid (NEAAs) in treatment ST5 (70 pc/m²) followed by ST2 (40 pc/m²), ST6 (80 pc/m²), ST3 (50 pc/m²), ST1 (30 pc/m²) and low in ST4 (60 pc/m²). Total 18 fatty acid were detected in all treatment. The results indicated that L. vannamei shrimp has higher volume of SFA, PUFA, MUFA. The total unsaturated fatty acids (USFAs) was higher in treatment ST2 with (66.15 μg/g of FAME) followed by ST3 (66.00μg/g of FAME), ST6 (65.58 μg/g of FAME), ST4 (63.04 μg/g of FAME), ST1 (62.75 μg/g of FAME) and ST5 (62.08 μg/g of FAME). Comparing all the treatment there is clear evident that stocking density affect the proximate, nutritive, amino acid and fatty acid level in L. vannamei cultured shrimp.