Effects of neem on fecundity and mortality of seven spotted ladybirds (Coccinella septempunctata) were evaluated under laboratory conditions in the Entomology laboratory at Paklihawa Agriculture campus of Rupandehi, Nepal from 13 March 2018 to 12 April 2018. The experiment was laid out according to a Complete Randomized Design (CRD) with four treatments and four replications each. Ladybirds were treated with the control, 2%, 3%, and 4% of neem solution. We found the effects of neem treatments on egg-laying, hatchability, and mortality to be non-significantly different. However, effects on male mortality and female mortality were significantly different. The impact of treatments on hatchability, mortality, and egg-laying were 31.04, 8.65, and 42.3 percent respectively. These results indicate that under the lab condition, neem treatment does not pose a negative effect on the insect.