The present study deals with Ichthyofaunal diversity of Kadana reservoir, Gujarat. Sampling was done at every month during July 2018 to February 2019. Total 32 species from 6 orders, 13 families and 22 genera were recorded during the present study. Cypriniformes was the dominant order in term of species diversity (12 species) followed by Siluriformes (10 species), Perciformes (6 species) and Synbranchiformes (2 species) and Osteoglossiformes and Beloniformes were represented by 1 species each. Dominant families were Cyprinidae (12species) followed by Bagridae (4 species), Siluridae and Channidae (3 species), Mastacembelidae (2 species), 1 species of Claridae, Heteropneustidae, Schilbeidae, Cichlidae, Ambassidae, Gobiidae, Belonidae and Notopteridae families. Total of 32 species of fishes were recorded during study period. Out of these 1 species comes under vulnerable status, 27 fish species comes under status of least concern, 1 fish species comes under data deficient status and 4 fish species comes under near threaten. During month of February highest fish diversity was found. Average fish diversity was found during month of July and lowest diversity was found during the month of November. This is first ever study on the fish diversity of Kadana reservoir and would help in explore the fish fauna of Kadana reservoir.