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Journal of Entomology and Zoology Studies
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P-ISSN: 2349-6800, E-ISSN: 2320-7078

Journal of Entomology and Zoology Studies

2019, Vol. 7, Issue 6
Isolation of Escherichia coli and Salmonella species in flies trapped at animal farm premises

Bhavna Wadaskar, Rahul Kolhe, Vikas Waskar, Mrunalini Pawade and Krishnendu Kundu

Present study was designed to explore the types of flies prevalent at food animal farms and their role in harboring the bacterial species namely Escherichia coli and Salmonella. Flies were trapped using indigenous traps from the cattle, sheep, goat, poultry farms and veterinary clinical complex. A total of 2187 flies were collected from the 36 pools during the months of April and May, 2019. All the collected flies were identified as house flies (Musca domestica) except one were identified as a blow fly (Calliphora erythrocephala). Processing of representative fly pool samples for isolation and identification of bacterial pathogens revealed 100% prevalence of E. coli in all the flies caught, whereas Salmonella was isolated from only 21 pools with a prevalence rate of 58.33%. It is thus concluded that flies prevalent at food animal farms are dominated by Musca domestica species and their role in harboring and transmission of E. coli and Salmonella species cannot be ruled out.
Fly collection from various food animal farms using the indigenously made fly trap
Fig. 1: Fly collection from various food animal farms using the indigenously made fly trap
Typical metallic sheen of <em>E. coli</em> on EMB agar and <em>E. coli </em>stained as gram negative pink rods
Fig. 2: Typical metallic sheen of E. coli on EMB agar and E. coli stained as gram negative pink rods
Black centered colonies of <em>Salmonella </em>spp. on XLD agar and <em>Salmonella</em> stained as gram negative pink rods
Fig. 3: Black centered colonies of Salmonella spp. on XLD agar and Salmonella stained as gram negative pink rods
Pages : 198-201 | 897 Views | 143 Downloads


Journal of Entomology and Zoology Studies Journal of Entomology and Zoology Studies
How to cite this article:
Bhavna Wadaskar, Rahul Kolhe, Vikas Waskar, Mrunalini Pawade, Krishnendu Kundu. Isolation of Escherichia coli and Salmonella species in flies trapped at animal farm premises. J Entomol Zool Stud 2019;7(6):198-201.

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