We studied the effects of different sorghum genotypes on developmental biology, and larval and adult behaviors of spotted stem borer, Chilo partellus. The test maize genotypes had significantly lower pupal weight, larval survival, adult emergence and fecundity as compared to susceptible check, Swarna. There was significant increase in premating period, decrease in pre-oviposition and oviposition periods, decrease in numbers of egg clusters and eggs per cluster, and increase in egg incubation period in C. partellus on these test genotypes as compared to those from susceptible check, Swarna. The studies on establishment and feeding behaviors of C. partellus larvae on different sorghum genotypes revealed that the neonate larvae took longer time to establish on the resistant sorghum genotypes, and the establishment on resistant sorghum genotypes was also significantly lower than on the susceptible genotype. Thus, the sorghum genotypes viz., ICSV 1, ICSV 700, ICSV 93046 and IS 18551 impart detrimental effects on the growth, development and progeny production of C. partellus and can be utilized in breeding to derive C. partellus resistant varieties of sorghum.