Molecular typing of Canine parvovirus-2 occuring in Nagpur by multiplex PCR
Magar SA, Warke SR, Tumlam UM, Bhojne GR and Ingle VC
Canine parvovirus is one of the major causes of haemorrhagic enteritis especially in young dog’s leads to high morbidity and fatality. The present study was designated for identification of Canine parvovirus in diarrhoeic dogs by Antigen detection kit, PCR and antigenic typing by employing Multiplex PCR.A total of 50 faecal samples from diarrheic dogs were screened for CPV antigen by Antigen detection test kit. Out of 50 samples 11 (22%) were found positive by Antigen detection kit test. All the 11 samples were found positive by Conventional PCR showing uniform band of 681 bp. All the 11 samples found positive by PCR were subjected to multiplex PCR assay with CPV-2, CPV-2a, CPV-2B AND CPV-2 c. It was inferred that CPV-2a were predominant antigenic type followed by CPV-2c and CPV-2 and negligible prevalence of CPV-2b.