author(s)
Rashmi R, N Prakash, HD Narayana swamy, M Narayana Swamy, D Rathnamma, Suguna Rao, A Sahadev, CR Santhosh, U Sunilchandra, Naveen Kumar S, Wilfred Ruban S, GP Kalmath, KR Anjan Kumar, Yathish HM and Prashantkumar Waghe

abstract
Phytocompounds are pleotropic in their function and preferentially target cancer cells without altering normal cells. In the present study, MTT [3-(4, 5- dimethylthiazolyl- 2)-2, 5- diphenyltetrazolium bromide] assay was conducted on MCF-7 and MDA-MB-231 cell lines at 48h incubation to determine percent cell viability. The IC₅₀ values of umbelliferone and piperine for MCF-7 were 15.56 and 17.83μM, respectively. Likewise, IC₅₀ values of umbelliferone and piperine for MDA-MB-231 were 10.31 and 14.28 μM, respectively. A combination of umbelliferone (IC₂₅) and piperine (IC₂₅) on MDA-MB-231 and MCF-7 showed percent cell viability of 8.24±0.01 and 15.12±0.01, respectively. This study suggests that the cytotoxicity of umbelliferone and piperine was dose-dependent. The in vitro cytotoxicity potential in terms of percent cell viability, early apoptosis, late apoptosis and G2M phase arrest of umbelliferone alone and in combination with piperine proved to be more effective on MDA-MB-231 cells.