author(s)
Rashmi R, N Prakash, HD Narayana Swamy, M Narayana Swamy, D Rathnamma, Suguna Rao, A Sahadev, CR Santhosh, U Sunilchandra, Naveen Kumar S, Wilfred Ruban S, GP Kalmath, H Dhanalakshmi, Leena G, AR Gomes, KR Anjan Kumar and Prashantkumar Waghe

abstract
Phytocompounds have played an important role as the source of effective anti-cancer agents. Over 60% of the currently used anticancer agents are derived from natural sources including plants, microorganisms and marine organisms. In the present study, oxidative stress indices such as DHE (dihydroethidium) and H₂DCFDA (2’, 7’-dichlorodihydrofluorescein diacetate) assays were conducted on MDA-MB-231 cell lines at 48h incubation. The phytocompounds such as umbelliferone and piperine at their respective IC₅₀ concentration and a combination of umbelliferone (IC₂₅) with piperine (IC₂₅) showed cytotoxicity due to increased production of reactive oxygen species indicated by an increase in fluorescence under DHE assay and an increase in mean fluorescence intensities by H₂DCFDA assay. Thus, the findings of our study may shed light on oxidative stress-mediated cytotoxicity in triple-negative breast cancer.