author(s)
A Nirwan, M Shekhawat, D Suthar, Diwakar and AK Kataria

abstract
Staphylococcus aureus is an important bacterial agent causing variety of infections in humans and animals. This organism causes diseases like skin infections, meningitis, endocarditis, septicemia, and toxic shock syndrome. Biofilm production is considered one of the critical virulent factors which enhance survival of the organism and hence contributes to the pathogenic capabilities of this organism. In our study, a total of 40 Staphylococcus aureus isolates were obtained from different sources like pus and skin of humans and animals and unprocessed meat samples. The isolates were obtained by conventional microbiological methods, confirmed genotypically by 23S rRNA ribotyping and Maldi-Tof MS. The confirmed S. aureus isolates were screened for detection biofilm formation by Congo red agar method and for the detection of two genes responsible for biofilm production, namely icaA and icaD by a simplex polymerase chain reaction. Out of 42.50% and 32.50% isolates were detected as healthy and intermediate biofilm produce by the CRA method, and 100% and 97.56% isolates were exposed to possess icaA and icaD gene respectively by PCR. The very high prevalence of two genes in the isolates indicates the high pathogenic ability of these organisms as these genes are responsible for intracellular adhesions.