RAPD-based genotyping of walking catfish, Clarias batrachus in a population of Bangladesh
Md. Faruque Miah, Toufiqur Rahman, Shahena Aktar Shipa and Anindita Chakrabarty
Genetic diversity of any fish is most important for their existing, breeding, production and management. In this study, intra-species genetic variation of a wild population of walking catfish, Clarias batrachus in Sylhet, Bangladesh was investigated. Four RAPD markers such as OPB-19, OPB-12, OPB-05 and OPF-14 were used to assess genetic variability among 8 individuals of C. batrachus genotypes. Less number of DNA bands was revealed by all the primers in experimental populations where a total of 132 bands were detected and all the markers were shown different levels of polymorphism. Intra-specific polymorphisms were recorded 60%, 55.56%, 40.91% and 30.77% in primer OPB-12, OPB-19, OPF-14 and OPB-05 respectively where average polymorphism was recorded 46.81%. Average gene diversity was found 0.19823. The genetic distance of experimental samples ranged from 0.6 to 1 and in an average 0.88, indicating higher genetic distances of this population. A genetic relationship among the individuals of the experimental population was constructed where 3 clades and 2 sub-clusters were recorded considering different linkage distances. Considering different genetic parameters genetic variability was found higher among this experimental population.
Md. Faruque Miah, Toufiqur Rahman, Shahena Aktar Shipa, Anindita Chakrabarty. RAPD-based genotyping of walking catfish, Clarias batrachus in a population of Bangladesh. J Entomol Zool Stud 2020;8(1):885-888.