author(s)
Jyothi V, Rajkishore Kumar and M Saifulla

abstract
An experiment was performed on “Assessment of molecular variability in Macrophomina phaseolina isolates of chickpea from different locations of India” The results revealed that, Molecular characterization of twenty isolates includes identification of M. phaseolina isolates with species specific primers MpKFI (5'- CCGCCAGAGGACTATCAAAC-3') and MpKRI (5' CGTCCGAAGCGAGGTGTATT-3') yielded single amplified product of 350 bp in all the isolates tested and the PCR amplified 18S rDNA region using ITS primers produced a band size of about 600bp. The results was further evident that, phylogenetic tree having the nucleotide sequences of ITS region. Clusters A and B indicating the variability in M. phaseolina and existence of two types of pathotype. In nature, new strains might have been arised by mutation, hybridization, differential cytoplasmic inheritance heterokaryosis and by parasexual life cycle. The pathogenic variability is essential tools for breeding disease resistance in a crop improvement programme. From ongoing discussion, it can be concluded that, potential pathogen often blessed with biodiversity within its population and variation in pathogen is desirable trait for its existence in nature. This variability among the pathogens underlies their diverse nature and ability to withstand the host environment.